Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor

A Chromatin-Based Mechanism for Limiting Divergent Noncoding Transcription

In addition to their annotated transcript, many eukaryotic mRNA promoters produce divergent noncoding transcripts. To define determinants of divergent promoter directionality, we used genomic replacement experiments. Sequences within noncoding transcripts specified their degradation pathways, and functional protein-coding transcripts could be produced in the divergent direction. To screen for m...

Repression of Meiotic Genes by Antisense Transcription and by Fkh2 Transcription Factor in Schizosaccharomyces pombe

In S. pombe, about 5% of genes are meiosis-specific and accumulate little or no mRNA during vegetative growth. Here we use Affymetrix tiling arrays to characterize transcripts in vegetative and meiotic cells. In vegetative cells, many meiotic genes, especially those induced in mid-meiosis, have abundant antisense transcripts. Disruption of the antisense transcription of three of these mid-meiot...

A transcription switch toggled by noncoding RNAs.

L ife is really getting complicated in the world of eukaryotic transcription. In the good old days, we were comfortable in the belief that transcription occurred primarily across the coding region of a gene, producing an mRNA for translation. However, over the past few years, this view has been turned topsy-turvy. Improved microarray and sequencing technologies have revealed that our picture of...

metabolic syndrome and mitochondrial transcription factor a

Induction of Heme Oxygenase -1 By Lipocalin 2 Mediated By Nf-Kb Transcription Factor

Purpose: Effect of lipocalin 2 on the expression of heme oxygenase I , II and NF-kB transcription factor was the purpose of this survey. Materials and Methods: Lcn2 was cloned to pcDNA3.1 plasmid by using genetic engineering method. The recombinant vector was transfected to CHO and HEK293T to establish stable cell expressing lipocalin 2. The presence of lipocalin 2 gene in these cells was confi...